For the quantitative determination of Human MDA concentrations.
Methode Type: Sandwich ELISA Detection
Quantity: 96 tests
Sample type： serum, plasma, Urine,tissue homogenates, cell culture supernates
Detection range : 0.3mmol/L-7 mmol/L
Sensitivity: 0.01 mmol/L
|Assay plate (12 × 8 coated Microwells)
|Chromogen Solution A
|Chromogen Solution B
Twelve months [see label on the outer box for the specific date]
The unopened kit shall be stored at [2-8 ℃]
For opened kit can be stored at [2-8 ℃] for up to 1 month. If not be used recently, the standard should be kept in -20 ℃
The kit takes out from the refrigeration should be balanced 15-30 minutes in the room temperature, if the coated
ELISA plates have not been used up after opening, the plate should be stored in sealed bag.
Washing buffer will Crystallization separation, it can be heated in water to dissolve.
Pipette sample with pipettors each step, and proofread its accuracy frequently to avoid the experimental error. Pipette
sample within 5 min, if the number of sample is big, recommend using multichannel pipettor.
If the testing material concentration is excessively high (The sample OD is higher than the first standard well)， please
dilute the sample (n-fold).
Adhesive Strip only limits the disposable use to avoid cross-contamination.
The substrate should evade the light to be preserved.
Please refer to the user instruction strictly, the test result determination must take the microtiter plate reader as a
The preparation of samples and all the reagents should refer to infective material process.
Do not mix reagents with those from other lots.
Manually washing method: shake away the remained liquid in the enzyme plates; place some bibulous papers on the
test-bed, and flap the plates on the upside down strongly. Inject at least 0.35ml after-dilution washing solution into the well,
and marinate 1~2 minutes. Repeat this process according to your requirements.
Automatic washing method: if there is automatic washing machine, it should only be used in the test when you are quite
familiar with its function and performance .